Invitrogen™ TOP10 Electrocomp™ Kit

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 10¹⁰ cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

Brand:  Invitrogen™ C66424

Product Code. 10226592

Description

TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 10¹⁰ cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. 

The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.

Genotype
F^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.

Specifications

• For Use With (Application): High-efficiency cloning and plasmid propagation
• Includes: Contains enough electrocompetent cells for 75 x 40μL rxns or 150 x 20μL rxns
• Quantity: 30 x 100 μL

For Research Use Only. Not for use in diagnostic procedures.