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Integrin beta 7, PE-Cyanine7, clone: FIB504, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-5867-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15588576

  • £74.42 / 25 tests


Estimated Shipment date
from Supplier 30-05-2018
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Description

Description

Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity.Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FIB504 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Integrin Beta 7
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P26010, P26011
Integrin beta-7, Gut homing receptor beta subunit, ITGB7
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
FIB504
PE-Cyanine7
ITGB7
Conjugated
ITGB7
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
3695
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