missing translation for 'onlineSavingsMsg'
Learn More

Integrin alpha 4 beta 7 (LPAM-1), PerCP-eFluor™ 710, clone: DATK32 (DATK-32), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5887-80

Code : NEW

Additional Details : Weight : 0.09500kg

 View more versions of this product

Product Code. 15589296

  • £78.81 / 25µg


EU Stock 3
Add to basket

Description

Description

Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154kDa alpha 4 subunit and the 130kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion.Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis.Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Integrin alpha 4 beta 7 (LPAM-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P26011, Q00651
ITGB7, Gut homing receptor beta subunit, Integrin alpha-4/beta-7, Peyer patches-specific homing receptor LPAM-1, Itga4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
DATK32 (DATK-32)
PerCP-eFluor 710
Itga4
Conjugated
Itga4, Itgb7
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
16401, 16421
Videos

For Research Use Only.