Thermo Scientific™ Imject™ Maleimide-Activated mcKLH and Kit

Perform hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens with this sulfhydryl-reactive mariculture KLH.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 77605

Imject Maleimide Activated Mariculture KLH10 mg

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg Transport: UN number : 3261 Chem class : 1a Pack group : III



Product Code. 10475685

Quantity Price
1 £ 411.0 / 10mg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Description Imject Maleimide Activated Mariculture KLH
Formulation Activated protein lyophilized in 10mM sodium phosphate, 150mM sodium chloride, 10mM EDTA, 50mM sucrose, pH 7.2
Quantity 10mg
Sufficient For 5 conjugation reactions, each with up to 2mg sulfhydryl-containing peptide
Kit Contents 0077605
Activation Maleimide
Format Lyophilized
Protein KLH, keyhole limpet hemocyanin
Item Description Activated mcKLH; 10mg

The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

  • Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit

  • Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation

  • Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

  • Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

  • High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture

  • Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin

  • Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization