Thermo Scientific™ Imject™ Maleimide-Activated BSA and Kit

Prepare hapten-carrier conjugates with cysteine-containing peptides and other thiolated antigens using this sulfhydryl-reactive bovine serum albumin.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 77116

IMJECT MALEIMIDE ACTIVATED BSA 2MG

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: CAS Number: 67423-45-4 Weight: 0.80000kg



Product Code. 11874061

Quantity Price
1 £ 93.25 / 2mg
Estimated Shipment date
from Supplier 12-12-2016
Add to basket


Description and Specification

Specification

Description Imject Maleimide Activated Bovine Serum Albumin
Formulation Activated protein lyophilized in 0.1M sodium phosphate, 150mM sodium chloride, 0.1M EDTA, 10mM sucrose, pH 7.2
Quantity 2mg
Sufficient For 1 conjugation reaction with up to 2mg sulfhydryl-containing peptide
Activation Maleimide
Format Lyophilized
Protein BSA, bovine serum albumin

Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

  • Convenient packages – choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit

  • Optimized activation – specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation

  • Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

  • Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

  • High-quality carrier protein – pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance

  • Immunogenic and soluble – moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization