IL-9 Monoclonal Antibody (RM9A4), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-8091-82

Product Code. 15351960

Description

Description: This monoclonal RM9A4 antibody reacts with mouse Interleukin-9 (IL-9). IL-9 is a 14 kDa cytokine originally named P40 and identified by its proliferative effects on T cell populations. The receptor, which is a heterodimer of the gamma chain portion of the IL-2 receptor and the IL-9R chain, activates Jak/STAT signaling pathways upon binding its ligand. Since the discovery of IL-9, numerous other functions have been observed. It induces Th17 and Treg differentiation in CD4+ T cells, IgE production in B cells, and the differentiation and proliferation of mast cells. IL-9 expression was initially observed in Th2 cells, but has since been found in Th17, eosinophil, and mast cells. Th9 cells, a newly discovered subset of CD4+ T cells, are characterized by the secretion of large amounts of IL-9 and IL-10. Th9 development is induced by stimulation of undifferentiated CD4+ with IL-4 and TGF beta. Th2 cells can also be driven towards a Th9 phenotype in the presence of TGF beta. Applications Reported: This RM9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This RM9A4 antibody has been tested by intracellular staining followed by flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

IL-9 Monoclonal antibody specifically detects IL-9 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IL-9
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P15247
• Gene Symbols: Il9
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16198
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: RM9A4
• Conjugate: eFluor 660
• Gene: Il9
• Gene Alias: cytokine P40; homolog of mouse T cell and mast cell growth factor 40; HP40; IL9; IL-9; ILN; Interleukin; interleukin 9; Interleukin9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.