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IL-9, PerCP-eFluor™ 710, clone: MH9D1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-7098-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15569346

  • £75.29 / 25 tests

Estimated Shipment date
from Supplier 25-04-2018
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Description: The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4.IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9D1 antibody is not blocked by the MH9A4 antibody, suggesting that the two antibodies bind to unique epitopes.Applications Reported: This MH9D1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MH9D1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th9-polarized human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.


PerCP-eFluor 710
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry (Intracellular Staining)
5μL (0.03μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-9, IL9, T-cell growth factor P40
IgG2a, kappa

For Research Use Only.