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IL-9, PE, clone: MH9D1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-7098-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15597086

  • £68.96 / 25 tests


EU Stock 1
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Description

Description

Description: The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4.IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9D1 antibody is not blocked by the MH9A4 antibody, suggesting that the two antibodies bind to unique epitopes.Applications Reported: This MH9D1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MH9D1 antibody has been pre-titrated and tested on restimulated Th2-polarized normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-9
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P15248
Interleukin-9
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
MH9D1
PE
IL9
Conjugated
IL9
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3578
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