IL-9, eFluor 660, clone: MH9A4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-7097-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The monoclonal antibody MH9A4 recognizes interleukin-9 (IL-9), a proinflammatory cytokine historically believed to be involved in type 2 immune responses. Recent evidence suggests IL-9 may instead be secreted by a distinct T-helper lineage called Th9. These cells can be derived from Th2 cells with TGF beta or differentiated directly from naive CD4+ T cells with TGF beta and IL-4.IL-9 is a member of the common cytokine receptor gamma chain-dependent family of cytokines which also includes IL-2, IL-4, IL-7, IL-15 and IL-21. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils and gut and airway epithelial cells have implicated IL-9 in asthma and other allergy-related disorders. Staining with the MH9A4 antibody is not blocked by the MH9D1 antibody, suggesting that the two antibodies bind to unique epitopes.Applications Reported: This MH9A4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MH9A4 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Flow CytometryþFlow Cytometry|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.