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IL-8 (1-77) (CXCL8), PerCP-eFluor™ 710, clone: 8CH, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-8088-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15539376

  • £226.06 / 100 tests


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: This 8CH monoclonal antibody reacts with human IL-8 (CXCL8), a pro-inflammatory CXC chemokine. It is synthesized as a 99 amino acid precursor protein that is further processed into one of four isoforms, with the most common being 72 or 77 amino acids in length. IL-8(77) is secreted primarily by endothelial cells and is thought to be a less potent neutrophil activator than the other forms. It is present at high levels during fetal development, where it mediates angiogenesis rather than inflammation. The predominant form present in adults is IL-8(72), which is expressed by monocytes, macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli, environmental stress, and steroid hormones. IL-8(72) is essential for the activation and recruitment of neutrophils to sites of inflammation, and has also been found to influence T cell migration. Signaling occurs through the G-protein coupled receptors CXCR1 or CXCR2. IL-8 transcripts are often upregulated in tumors, and it is associated with tumor angiogenesis and metastasis.Applications Reported: This 8CH antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 8CH antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-8
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P10145
alveolar macrophage chemotactic factor I, beta endothelial cell-derived neutrophil activating peptide, beta-thromboglobulin-like protein, chemokine (C-X-C motif) ligand 8, emoctakin, granulocyte chemotactic protein 1, interleukin 8, interleukin-8, lung giant cell carcinoma-derived chemotactic protein, lymphocyte derived neutrophil activating peptide, lymphocyte-derived neutrophil-activating factor, monocyte-derived neutrophil chemotactic factor, monocyte-derived neutrophil-activating peptide, neutrophil-activating peptide 1, small inducible cytokine subfamily B, member 8, T cell chemotactic factor, T-cell chemotactic factor, tumor necrosis factor-induced gene 1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
8CH
PerCP-eFluor 710
CXCL8
Conjugated
CXCL8
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3576
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