IL-6, PE, clone: MP5-20F3, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-7061-81
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MP5-20F3 antibody is offered in 2 formats: -μg size: has been tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at test size: 5μL (0.125μg) per test.The pre-titrated test size contains BSA and/or gelatin for protein stabilization.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Interleukin-6, BSF2, HGF, HSF, IFNB2, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.