IL-6, Functional Grade, clone: MP5-20F3, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7061-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.Applications Reported: This MP5-20F3 antibody has been reported for use in cytokine neutralization, ELISA capture antibody, and ELISPOT capture antibody.Applications Tested: The Functional Grade Purified MP5-20F3 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-6 bioactivity. The MP5-20F3 antibody at 0.03 μg/mL has been found to inhibit by 50% the biological effects of 0.01 ng/mL mouse IL-6 in an B9 cell proliferation assay. Detailed information and protocols about can be found in the BestProtocols® section.The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-6 (IL-6) in combination with the biotinylated MP5-32C11 (cat. 13-7062) antibody for detection and recombinant mouse IL-6 (cat. 14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Interleukin-6, BSF2, HGF, HSF, IFNB2, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor|
|Cytokine NeutralizationþELISAþDot blot|
|Store at 2-8°C.|
For Research Use Only.