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IL-6, clone: MP5-20F3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7061-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15287997

  • £151.64 / 500µg


EU Stock 2
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Disclaimers:

For Research Use Only.


Description

Description

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.Applications Reported: This MP5-20F3 antibody has been reported for use in ELISA capture antibody and ELISPOT capture antibody.Applications Tested: The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the biotin MP5-32C11 (cat. 13-7062) antibody for detection and recombinant mouse IL-6 (cat. 14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-6
MP5-20F3
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
ELISPOT Capture Antibody
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-6
IgG1
RUO
Primary
Murine
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