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IL-6, Alexa Fluor 700, clone: MQ2-13A5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-7069-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15570367

  • £89.74 / 25 tests


Estimated Shipment date
from Supplier 01-06-2018
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Description

Description

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28kDa cytokine secreted by a variety of cell types.Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-6
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P05231
Interleukin-6, BSF2, HGF, HSF, IFNB2, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
MQ2-13A5
Alexa Fluor 700
IL6
Conjugated
IL6
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3569
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