IL-6, Alexa Fluor 700, clone: MQ2-13A5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-7069-41

Product Code. 15570367

Description

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28kDa cytokine secreted by a variety of cell types.Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: IL-6
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05231
• Gene Alias: Interleukin-6, BSF2, HGF, HSF, IFNB2, IL-6, B-cell stimulatory factor 2, BSF-2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: MQ2-13A5
• Conjugate: Alexa Fluor 700
• Gene: IL6
• Format: Conjugated
• Gene Symbols: IL6
• Isotype: IgG1, kappa
• Quantity: 25 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 3569

For Research Use Only.