IL-6 Monoclonal Antibody (MQ2-13A5), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15580367	Affymetrix eBioscience 56-7069-42	100 Tests

Description

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types. Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

IL-6 Monoclonal antibody specifically detects IL-6 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IL-6
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P05231
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: MQ2-13A5
• Applications: Flow Cytometry
• Conjugate: Alexa Fluor 700
• Host Species: Rat
• Target Species: Human
• Gene ID (Entrez): 3569
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.