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IL-5, clone: TRFK5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7052-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15267997

  • £151.64 / 500µg


EU Stock 1
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Description

Description

Description: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5). The TRFK5 antibody is a neutralizing antibody. Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.Applications Reported: The TRFK5 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-5 bioactivity, IHC, and intracellular staining for flow cytometric analysis.Applications Tested: The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-5 in combination with the biotin TRFK4 (13-7051) antibody for detection and recombinant mouse IL-5 (14-8051) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The TRFK5 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-5 in combination with the biotinylated JES1-5A10 (13-7059) antibody for detection and recombinant human IL-5 (14-80591) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.The Functional Grade purified TRFK5 antibody has been tested for neutralization of IL-5 bioactivity.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-5
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04401, P05113
Interleukin-5, EDF, IL-5, TRF, T-cell replacing factor, TRF, Eosinophil differentiation factor, B cell differentiation factor I
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
TRFK5
Unconjugated
IL5
Purified
Il5
IgG1, kappa
500μg
Store at 2-8°C.
Primary
3567
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