IL-4, PE-Cyanine7, clone: BVD6-24G2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 25-7042-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14kDa cytokine secreted by Th2 cells.Applications Reported: This BVD6-24G2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This BVD6-24G2 antibody is offered in 2 formats: - μg size: has been tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes cultures. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.