IL-4, PE, clone: 11B11, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-7041-81
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.Applications Reported: This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 11B11 antibody is offered in 2 formats: -μg size: has been tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes. This can be used at less than or equal to 0.125μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining of Th2 polarized and stimulated mouse splenocytes. This can be used at 5μL (0.06μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|0.2mg/mL; 5μL (0.06μg)/test|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
For Research Use Only.