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IL-4, clone: 11B11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7041-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15257987

  • £49.88 / 50µg


EU Stock 1
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Description

Description

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.Applications Reported: The 11B11 antibody has been reported for use in ELISA capture, ELISPOT capture, and neutralization of IL-4 bioactivity.Applications Tested: The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the biotin BVD6-24G2 (cat. 13-7042) antibody for detection and recombinant mouse IL-4 (cat. 14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-4
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P07750
Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
ELISAþDot blot
11B11
Unconjugated
Il4
Purified
Il4
IgG1, kappa
50μg
Store at 2-8°C.
Primary
16189
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