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IL-4, PE-Cyanine7, clone: 8D4-8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-7049-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15351000

  • £189.82 / 100µg


EU Stock 9
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Description

Description

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19kDa cytokine secreted by Th2 cells.Applications Reported: The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.Applications Tested: This 8D4-8 antibody is offered in 2 formats: -μg size: has been tested by intracellular flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P05112
Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
8D4-8
PE-Cyanine7
IL4
Conjugated
IL4
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
3565
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