IL-4, clone: 8D4-8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7049-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19kDa cytokine secreted by Th2 cells.Applications Reported: The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.Applications Tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (13-7048) antibody for detection and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:- μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402|
|Store at 2-8°C.|
For Research Use Only.