missing translation for 'onlineSavingsMsg'
Learn More

IL-4, clone: 8D4-8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7049-81

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15217997

  • £49.88 / 50µg


EU Stock 3
Add to basket

Description

Description

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19kDa cytokine secreted by Th2 cells.Applications Reported: The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.Applications Tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (13-7048) antibody for detection and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:- μg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-4
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P05112
Interleukin-4, BSF1, IL-4, Lymphocyte stimulatory factor 1, MGC79402
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ELISA
8D4-8
Unconjugated
IL4
Purified
IL4
IgG1, kappa
50μg
Store at 2-8°C.
Primary
3565
Videos

For Research Use Only.