IL-31 Monoclonal Antibody (31SNEZE), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9319-41

Product Code. 15590087

Description

Description: This 31SNEZE monoclonal antibody reacts with human IL-31. Interleukin-31 (IL-31) is a recently-discovered member of the IL-6 family of cytokines. It is expressed by CD4+ T cells, particularly those with a Th2 phenotype, and mast cells. The IL-31 receptor is a heterodimer composed of one IL-31RA and one OSMR beta subunit. This receptor is constitutively expressed by various cells types, including macrophages, monocytes, eosinophils, keratinocytes, and epithelial cells. IL-31 receptor signaling leads to STAT3 activation and promotes the release of proinflammatory cytokines and chemokines, such as IL-1 beta, IL-6, IL-8, CXCL1, and CCL2. Over-expression of IL-31 is observed in patients with allergic asthma, allergic rhinitis, and atopic dermatitis, with a positive correlation between circulating levels and disease severity. Applications Reported: This 31SNEZE antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 31SNEZE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of human peripheral blood lymphocytes. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered. Interleukin 31, or IL-31 is a cytokine secreted by differentiated CD4+ T helper type 2 (Th2) cells. It is also released during inflammation of the skin, by macrophages and T cells. Because IL-31 is secreted by activated Th2 cells, it has a role in host defense against infection and may be involved in asthma, allergy and different types of bowel diseases.

Specifications

• Antigen: IL-31
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q6EBC2
• Gene Symbols: IL31
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 386653
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 31SNEZE
• Conjugate: eFluor 660
• Gene: IL31
• Gene Alias: Interleukin-31
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.