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IL-23 p19, eFluor 660, clone: fc23cpg, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-7023-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15341940

  • £93.30 / 25µg


Estimated Shipment date
from Supplier 24-09-2018
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Description

Description

Description: This fc23cpg monoclonal antibody reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine made up of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells. The receptor shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. Binding of IL-23 to its receptor activates Jak/STAT pathways similarly to IL-12, but the two cytokines exhibit distinct functions. Unlike IL-12, which induces T-bet and Th1 differentiation, IL-23 signaling results in RORgammat expression and supports CD4^+ T cells with the Th17 phenotypeDendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.Applications Reported: This fc23cpg antibody has been reported for use by intracellular staining and flow cytometric analysis.Applications Tested: This fc23cpg antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-23 p19
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9EQ14
IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
fc23cpg
eFluor 660
Il23a
Liquid
Il23a
IgG1, kappa, kappa
25μg
4° C, store in dark, DO NOT FREEZE!
Primary
83430
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