IL-23 p19, Functional Grade, clone: HNU2319, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5236-85

Product Code. 15208647

Description

Description: The HNU2319 monoclonal antibody is specific to the p19 subunit of human IL-23, a heterodimeric cytokine made up of two covalently linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as the beta-glucan receptor Dectin-1. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. In contrast, the mouse IL-23 biological activities are more distinctly different than those of mouse IL-12 suggesting the signaling or response elements (receptors) differ between species. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to IL-23. Signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which drives the differentiation of CD4^+ T-cells towards the Th17 phenotype. The monoclonal antibody HNU2319 neutralizes the bioactivity of IL-23 without affecting IL-12.Applications Reported: The monoclonal antibody HNU2319 reacts with the p19 subunit of human IL-23 and inhibits its bioactivity.Applications Tested: The ND50 of HNU2319, as determined by the neutralization of mouse IL-17A induction in balb/c splenocytes by recombinant human IL-23, is 0.2-0.4 μg/mL in the presence of 2 ng/mL IL-23. This antibody is specific to the p19 subunit of IL-23, and will not affect the bioactivity of IL-12. The neutralization dose of this antibody will vary depending on cell type, assay method, and concentration of cytokine.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.

Specifications

• Antigen: IL-23 p19
• Classification: Monoclonal
• Concentration: 1mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q9NPF7
• Gene Alias: IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Cytokine Neutralization
• Clone: HNU2319
• Conjugate: Functional Grade
• Gene: IL23A
• Format: Purified
• Gene Symbols: IL23A
• Isotype: IgG1, kappa
• Quantity: 500μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 51561

For Research Use Only.