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IL-23 p19, Functional Grade, clone: HNU2319, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5236-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208647

  • £175.12 / 500µg


Estimated Shipment
24-01-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The HNU2319 monoclonal antibody is specific to the p19 subunit of human IL-23, a heterodimeric cytokine made up of two covalently linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as the beta-glucan receptor Dectin-1. Human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by naive and memory T cells, as compared to IL-12. In contrast, the mouse IL-23 biological activities are more distinctly different than those of mouse IL-12 suggesting the signaling or response elements (receptors) differ between species. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to IL-23. Signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which drives the differentiation of CD4^+ T-cells towards the Th17 phenotype. The monoclonal antibody HNU2319 neutralizes the bioactivity of IL-23 without affecting IL-12.Applications Reported: The monoclonal antibody HNU2319 reacts with the p19 subunit of human IL-23 and inhibits its bioactivity.Applications Tested: The ND50 of HNU2319, as determined by the neutralization of mouse IL-17A induction in balb/c splenocytes by recombinant human IL-23, is 0.2-0.4 μg/mL in the presence of 2 ng/mL IL-23. This antibody is specific to the p19 subunit of IL-23, and will not affect the bioactivity of IL-12. The neutralization dose of this antibody will vary depending on cell type, assay method, and concentration of cytokine.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

IL-23 p19
HNU2319
Unconjugated
Purified
Murine
500μg
Store at 2-8°C.
Monoclonal
Cytokine Neutralization
1mg/mL
aqueous buffer, no sodium azide
Interleukin 23 p19, IL23 p19, IL23p19, IL-23p19
IgG1
RUO
Primary
Human
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