IL-23 p19, PerCP-eFluor™ 710, clone: 23dcdp, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 46-7823-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12R\beta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in ROR\gammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells. Dendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the \beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
For Research Use Only.