IL-23 p19, eFluor 660, clone: 23dcdp, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 50-7823-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: This 23dcdp monoclonal antibody reacts with the p19 subunit of human IL-23. This heterodimeric cytokine is composed of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. The IL-23 receptor is also heterodimeric and shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. IL-23R signaling occurs through the Jak/STAT pathway and results in RORgammat expression, which promotes maintenance and proliferation of T helper 17 (Th17) cells.Dendritic cells and macrophages produce high levels of p40 and low levels of IL-23 in response to TLR2, TLR4, and TLR8 ligands and agonists of the beta-glucan receptor, Dectin-1. This is due to the presence of monomer and dimer forms of p40, while only a small portion associates with p19 to form IL-23. Our studies suggest donor variability, expression kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.Applications Reported: This 23dcdp antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 23dcdp antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of cultured human dendritic cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
For Research Use Only.