IL-22, Functional Grade, clone: IL22JOP, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7222-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 cells, NK cells, and other T cells. Compared to IL-6 or TGF beta, IL-23 can induce greater levels of IL-22 in in vitro-differentiated Th17 cells. This observation suggests that IL-22 may be secreted by more fully differentiated Th17 cells in vivo. Recently, it was demonstrated that IL-22 could protect hosts from bacterial infection of the lungs and gut. Moreover, it has been reported that anti-CD3/CD28-induced production of IL-22 by PBMCs was elevated significantly in asthma patients compared to control patients. Flow cytometric analysis also showed that the frequencies of IL-17+IL-22+ CD4 T cells were increased in PBMCs from patients with ankylosing spondylitis and rheumatoid arthritis.IL22JOP is published to recognize rhesus IL-22.Applications Reported: The monoclonal antibody IL22JOP reacts with and inhibits the bioactivity of human and mouse IL-22.Applications Tested: The ND50 of IL22JOP, as determined by the inhibition of IL-10 induction in COLO205 cells by IL-22, is 10-40 ng/mL, in the presence of 0.2 ng/mL recombinant human or mouse IL-22. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|Store at 2-8°C.|
|aqueous buffer, no sodium azide|