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IL-22, PE-Cyanine7, clone: 22URTI, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-7229-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15508646

  • £107.53 / 25 tests


EU Stock 9
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Description

Description

Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.Applications Reported: This 22URTI antibody has been reported for use in flow cytometric analysis.Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral CD4 cells cultured in Th17 polarizing conditions. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-22
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9GZX6
Interleukin-22, IL22, IL-10-related T-cell-derived-inducible factor, IL-TIF
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow CytometryĆ¾Flow Cytometry
22URTI
PE-Cyanine7
IL22
Conjugated
IL22
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
50616
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