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IL-22, FITC, clone: 22URTI, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-7229-42
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of restimulated, Th17-polarized CD4+ human peripheral blood mononuclear cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
IL-22 | |
Monoclonal | |
5μl/Test | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Q9GZX6 | |
Interleukin-22, IL22, IL-10-related T-cell-derived-inducible factor, IL-TIF | |
Murine | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Human |
Flow Cytometry | |
22URTI | |
FITC | |
IL22 | |
Conjugated | |
IL22 | |
IgG1, kappa | |
100 Tests | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
50616 |
For Research Use Only.