missing translation for 'onlineSavingsMsg'
Learn More

IL-22, FITC, clone: 22URTI, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-7229-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15576596

  • £115.65 / 25 tests


EU Stock 1
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of restimulated, Th17-polarized CD4+ human peripheral blood mononuclear cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-22
22URTI
FITC
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-22
IgG1
RUO
Primary
Human
Videos