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IL-22, eFluor 450, clone: 22URTI, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7229-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519816

  • £76.42 / 25 tests


Estimated Shipment date
from Supplier 23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17-polarized normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-22
22URTI
eFluor 450
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (0.06μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-22
IgG1
RUO
Primary
Human
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