IL-22, eFluor 450, clone: 22URTI, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-7229-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 22URTI monoclonal antibody reacts with human interleukin(IL)-22. IL-22 is a 20kDa member of the IL-10 cytokine family that is secreted primarily by Th17 and NK cells. Nevertheless, other T cells have also been shown to produce IL-22. In in vitro Th17 cultures, induction of IL-22 expression is greater in response to IL-23 than IL-6 or TGF beta, suggesting that this cytokine may be secreted by more fully differentiated Th17 cells in vivo. A heterodimer consisting of IL-10R2 and IL-22R1 serves as the receptor for IL-22.Applications Reported: This 22URTI antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 22URTI antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17-polarized normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Interleukin-22, IL22, IL-10-related T-cell-derived-inducible factor, IL-TIF|
|Flow CytometryþFlow Cytometry|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.