IL-21, clone: eBio3A3-N2 (3A3-N2), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7219-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.Applications Reported: The eBio3A3-N2 antibody has been reported for use in ELISA capture of IL-21 analysis.Applications Tested: The eBio3A3-N2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-21 (IL-21) in combination with the biotin eBio2B2-G20 (cat.13-7218) antibody for detection and recombinant standard human IL-21 (cat. 39-8219) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 60 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|