IL-2 Monoclonal Antibody (JES6-5H4), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-7021-80

Product Code. 15382060

Description

Description: The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. Applications Reported: The JES6-5H4 antibody has been reported useful for ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis. Applications Tested: The Alexa Fluor™ 488 JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

Specifications

• Antigen: IL-2
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P04351
• Gene Symbols: Il2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16183
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: JES6-5H4
• Conjugate: Alexa Fluor 488
• Gene: Il2
• Gene Alias: Interleukin-2, T-cell growth factor, TCGF, Aldesleukin
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.