IL-17F Rat anti-Mouse, Clone: RN17, Functional Grade, eBioscience™

Rat Monoclonal Antibody, Functional Grade

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 16-7473-82

100UG:ANTI-MOUSE IL-17F FUNCTIONAL GRADE PURIFIED

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15288807

Quantity Price
1 £ 138.0 / 100µg
Estimated Shipment date
from Supplier 08-12-2016
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Description and Specification

Specification

Antigen IL-17F
Applications Cytokine Neutralization
Clone RN17
Concentration 1mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, no sodium azide
Host Species Rat
Isotype IgG1
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Avoid repeated freeze/thaw cycles. KEEP CONTENTS STERILE.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The monoclonal antibody RN17 reacts with and inhibits the bioactivity of mouse IL-17F. IL-17F is a 37kD homodimer of the IL-17 family and a signature Th17 marker. Of all the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity), and the two genes are located in the same chromosomal region. Recent studies have demonstrated coordinated regulation of IL-17A and IL-17F during Th17 differentiation. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes, specifically by activated human CD4+ T cells. In addition to IL-17A, differentiated Th17 cells also produce IL-17F and IL-22 upon re-activation. Like IL-17A, IL-17F has been linked with inflammatory diseases. IL-17F and IL-17A expression has been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. IL-17F treatment of airway epithelium, vein endothelial cells, and fibroblasts has been reported to induce expression of IL-6, IL-8, GRO-α, ENA-78, TGF-β, MCP-1, G-CSF, GM-CSF, and ICAM-1.

Like IL-17A, IL-17F is a disulfide-linked homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-α secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). In the mouse, the IL-17A/F heterodimer (alone or in synergy with TNF-α) was found to regulate the expression of IL-6 and KC (mouse homolog of human GRO-α); this was found to be dependent on IL-17RA and TRAF6.