missing translation for 'onlineSavingsMsg'
Learn More

IL-17F, PE-Cyanine7, clone: SHLR17, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-7169-42

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15558636

  • £205.37 / 100 tests

EU Stock 7
Add to basket



Description: The SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F. IL-17F is a homodimeric, 34kDa member of the IL-17 family, which includes IL-17(A), B, C, D, E (IL-25). Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely. IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well. \i{In vitro\i} cultured Th17 cells have been shown to express IL-17F and IL-17A homodimers and IL-17A/F heterodimers depending on the culture conditions and their differentiation state. A heterodimer of IL-17RA and IL-17RC has been shown to bind IL-17F, resulting in target cell-secretion of pro-inflammatory cytokines and chemokines, as well as neutrophil recruitment.Applications Reported: This SHLR17 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SHLR17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17 polarized human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Flow Cytometry (Intracellular Staining)
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
IgG1, kappa

For Research Use Only.