IL-17F, Functional Grade, clone: SHLR17, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7169-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: This SHLR17 monoclonal antibody reacts with human interleukin (IL)-17F. IL-17F is a homodimeric, 34kDa member of the IL-17 family, which includes IL-17(A), B, C, D, E (IL-25). Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely. IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well. In vitro cultured Th17 cells have been shown to express IL-17F and IL-17A homodimers and IL-17A/F heterodimers depending on the culture conditions and their differentiation state. A heterodimer of IL-17RA and IL-17RC has been shown to bind IL-17F, resulting in target cell-secretion of pro-inflammatory cytokines and chemokines, as well as neutrophil recruitment.This SHLR17 monoclonal antibody neutralizes the bioactivity of IL-17F homodimers without affecting IL-17A/F heterodimers.Applications Reported: This SHLR17 monoclonal antibody inhibits the activity of human IL-17F.Applications Tested: The ND50 of SHLR17, as measured by the inhibition of mouse IL-6 induction in NIH/3T3 cells, is 0.5-4μg/mL in the presence of 1μg/mL of Human IL-17F Recombinant Protein. Neutralization dose will vary depending on assay method, cytokine concentration, and cell type. This antibody should be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|Store at 2-8°C.|
|aqueous buffer, no sodium azide|