IL-17F Monoclonal Antibody (H17F10A7), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7479-82

Product Code. 15208087

Description

Description: The monoclonal antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7. Applications Reported: This H17F10A7 antibody has been reported for use in immunoblotting (WB). Applications Tested: This H17F10A7 antibody has been tested at 1-2μg/mL by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.

IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

Specifications

• Antigen: IL-17F
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q96PD4
• Gene Symbols: IL17F
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 112744
• Content And Storage: 4° C
• Form: Liquid

• Applications: Western Blot
• Clone: H17F10A7
• Conjugate: Unconjugated
• Gene: IL17F
• Gene Alias: C87042; CANDF6; cytokine ML-1; IL17F; IL-17F; IL-24; ILN; Interleukin; interleukin 17F; Interleukin17F; interleukin-17F; interleukin-24; ML1; ML-1; mutant IL-17F
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

For Research Use Only.