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IL-17F, clone: H17F10A7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7479-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298077

  • £82.04 / 25µg


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Description

Description

Description: The monoclonal antibody H17F10A7 reacts with human IL-17F, a member of the IL-17 family of pro-inflammatory cytokines. Like IL-17A, IL-17F is a disulfide-linked, homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-β, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-β and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). These heterodimers can be detected by immunoprecipitation with eBio64CAP17 anti-IL-17A monoclonal antibody followed by immunoblot with H17F10A7.Applications Reported: This H17F10A7 antibody has been reported for use in immunoblotting (WB).Applications Tested: This H17F10A7 antibody has been tested at 1-2μg/mL by western blot analysis of cultured, activated Th17 cells, where it detects a band of approximately 18kDa.It is recommended that H17F10A7 be used under reducing conditions only.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-17F
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q96PD4
IL-17F, ML-1, ML1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Western Blot
H17F10A7
Unconjugated
IL17F
Purified
IL17F
IgG1
25μg
Store at 2-8°C.
Primary
112744
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