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IL-17AF, PE, clone: B8KN8R, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9171-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15587176

  • £93.27 / 25µg


Estimated Shipment date
from Supplier 27-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The B8KN8R monoclonal antibody reacts with mouse interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.Applications Reported: This B8KN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This B8KN8R antibody has been tested by intracellular staining and flow cytometric analysis of Th17-polarized mouse splenocytes, using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocls: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resource Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-17AF
B8KN8R
PE
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-17AF
IgG2a
RUO
Primary
Murine
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