IL-17A, Functional Grade, clone: eBio64CAP17, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7178-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio64CAP17 ab reacts with Hu IL-17A; cross react with Rhesus monkey IL-17A, as verified by intracellular staining. The eBio64CAP17 ab is a neutralizing ab and has been shown to have no reactivity to Hu IL-17F. Reactivity to other members of the IL-17 family has not been evaluated. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. cDNA encoding Hu IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent aa identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63% with Ms CTLA-8. Hu IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the ICAM-1 in Hu fibroblasts. Hu IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of Hu IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Ms, rat, and Hu IL-17A can induce IL-6 secretion in Ms stromal cells, indicating that all homologs can recognize the Ms IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Additionally, activated Hu CD4+ T cells have been found to produce the IL-17A/F heterodimer, as well as the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all 3 were found to induce GRO-a secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). eBio64CAP17 can be used to detect IL-17 heterodimers by immunoprecipitation followed by immunoblot withH17F10A7 anti-IL17F monoclonal ab.The eBio64CAP17 has been shown to react to rhesus and marmoset primates.Applications Reported: The eBio64CAP17 ab has been reported for use as the capture ab in a Hu IL-17A ELISA and ELISPOT assay, for neutralization of IL-17A bioactivity, and for intracellular staining of IL-17A-producing cells.Applications Tested: The Affinity Purified eBio64CAP17 ab has been tested as the capture ab in a sandwich ELISA for measurement of Hu IL-17A protein levels, in combination with the biotinylated eBio64DEC17 ab (13-7179) for detection and recombinant Hu IL-17A (14-8179) as the standard. A suitable range of concentrations of this ab for ELISA capture is 0.5 - 2.0ug/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. The Functional Grade Purified eBio64CAP17 ab has been tested by LAL assay to verify lowest endotoxin levels and has been tested for neutralization of IL-17Abioactivity and for ELISPOT capture. For in vitro neutralization, the eBio64CAP17 ab at less than or equal to 63 ng/mL has been found to neutralize by 50% the biological effect of 5 ng/mL Hu IL-17A, based on induction of IL-6 secretion by the NHDF cell line. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. It is recommended that the ab be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug ab as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001ng/μg ab, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Store at 2-8°C.|
For Research Use Only.