IL-15, Functional Grade, clone: AIO.3, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-7154-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The AIO.3 antibody reacts with mouse IL-15. This is a neutralizing antibody. Mouse IL-15 is a 14-15kDa factor, previously known as IL-T. IL-15 is produced by a variety of cells and has a role in the T cell-mediated immune response and the central nervous system. IL-15 binds to the beta and gamma subunits of the IL-2 receptor; however, these two cytokines have distinct biological activities. IL-15 is a specific maturation factor for NK cells and mimics the BM microenvironment for NK cell development in vitro. It is reported that IL-15 delivers an anti-apoptotic and survival signal for NK, T, and B cells.Applications Reported: The AIO.3 antibody is reported for use in neutralization of mouse IL-15 bioactivity.Applications Tested: The Functional Grade Purified AIO.3 antibody has been tested by LAL assay to verify low endotoxin levels of < 0.01 ng/ug and in bioassay for neutralization of mouse IL-15 bioactivity. The AIO.3 antibody at less than or equal to 0.39 μg/mL has been found to inhibit by 50% the biological effects of 50 ng/mL mouse IL-15 (14-8151) in a CTLL2 proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|Cytokine NeutralizationþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.