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IL-13, PE-eFluor 610, clone: eBio13A, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 61-7133-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15520557

  • £80.39 / 25µg


Estimated Shipment date
from Supplier 20-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing. In cancer models it has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-13
eBio13A
PE-eFluor 610
Conjugated
Rat
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry (Intracellular Staining)
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-13
IgG1
RUO
Primary
Murine
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