IL-13, clone: eBio13A, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-7133-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)Applications Reported: The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.Applications Tested: The eBio13A antibody has been tested as the capture antibody in a sandwich ELISA (and ELISPOT) for analysis of mouse IL-13, in combination with the biotinylated eBio1316H antibody for detection (13-7135) and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate. The Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|Store at 2-8°C.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|