IL-12 Mouse anti-Mouse, Clone: MMp35A1.6 (A1.6), Functional Grade, eBioscience™

Mouse Monoclonal Antibody, Functional Grade

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 16-7360-85

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15587816

Quantity Price
1 £ 207.27 / 500µg
Estimated Shipment date
from Supplier 04-04-2017
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Description and Specification

Specification

Formulation aqueous buffer, no sodium azide
Storage Requirements Store at 2-8°C.
Gene Alias Interleukin-12 p35
Antigen IL-12
Isotype IgG2a
Regulatory Status RUO
Clone MMp35A1.6 (A1.6)
Species Reactivity Mouse
Concentration 1mg/mL
Host Species Mouse
Primary or Secondary Primary
Conjugate Unlabeled
Quantity 500μg
Monoclonal or Polyclonal Monoclonal
Format Purified
Applications Functional Assay

The MMp35A1.6 (A1.6) antibody reacts with the p35 subunit of mouse interleukin-12 (IL-12) and inhibits IL-12 bioactivity (in vitro and in vivo) but not the bioactivity of IL-35. p35 is a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.

The p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.

More recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naïve CD4+ cells cultured in its presence.