IL-12 Mouse anti-Mouse, Clone: MMp35A1.6 (A1.6), Functional Grade, eBioscience™

Mouse Monoclonal Antibody, Functional Grade

Brand: Affymetrix eBioscience

Manufacturer Part Number: 16-7360-82

100UG Anti-Mouse IL-12 p35 Functional Grade Purified

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15577816

Quantity Price
1 £129.50 / 100µg
Estimated Shipment date
from Supplier 26-10-2016
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Description and Specification


Antigen IL-12
Applications Functional Assay
Clone MMp35A1.6 (A1.6)
Concentration 1mg/mL
Conjugate Unlabeled
Format Purified
Formulation aqueous buffer, no sodium azide
Gene Alias Interleukin-12 p35
Host Species Mouse
Isotype IgG2a
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The MMp35A1.6 (A1.6) antibody reacts with the p35 subunit of mouse interleukin-12 (IL-12) and inhibits IL-12 bioactivity (in vitro and in vivo) but not the bioactivity of IL-35. p35 is a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.

The p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.

More recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naïve CD4+ cells cultured in its presence.