IL-12/IL-23 Rat anti-Mouse, eFluor 660, Clone: 4D10p35, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-7352-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionThis 4D10p35 monoclonal antibody reacts with mouse p35, a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.
The p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.
More recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naïve CD4+ cells cultured in its presence.
Testing of 4D10p35 in the context of IL-35 has not been evaluated. For additional information, please contact technical support.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow Cytometry (Intracellular Staining)|