IL-12/IL-23 p40 Rat anti-Mouse, eFluor™ 450, Clone: C17.8, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 48-7123-80
Code : NEW
Additional Details : Weight : 0.09500kg
Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells. Applications Reported: This C17.8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This C17.8 antibody has been tested inintracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered. IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintece and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IL12B; Il12p40; interleukin 12B; RP23-388G23.1; Il-12b; Il-12p40; p40; IL-12 p40; IL-23 subunit p40; IL12P40|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.