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IL-12/IL-23 p40, clone: C15.6, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7125-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288017

  • £56.02 / 50µg


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Description

Description

Description: The C15.6 antibody reacts with the p40 subunit of mouse interleukin-12 and IL-23. IL-12 is a 70kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated B cells and macrophages. IL-23 is a structurally and biologically-related cytokine comprised of the same p40 subunit and a p19 subunit. For optimal detection of total IL-12 p40 (monomer, heterodimer, homodimer) by ELISA, a combination of purified C15.6 antibody for capture and biotinylated C17.8 antibody for detection is recommended. Fluorochrome conjugated C15.6 antibody is effective for staining intracellular IL-12.Applications Reported: The C15.6 antibody has been reported for use in ELISA.Applications Tested: The C15.6 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IL-12 (p40 monomer or p40 dimerized with p35) or mouse IL-23 (p40 dimerized with p19) in combination with the biotin C17.8 (13-7123) antibody for detection and recombinant mouse IL-12 p70 (14-8121) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 μg/mL. A standard curve consisting of doubling dilutions of the recombinant IL-12 p70 standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-12/IL-23
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P43432
IL12B, Il12p40, interleukin 12B, RP23-388G23.1, Il-12b, Il-12p40, p40, IL-12 p40, IL-23 subunit p40, IL12P40
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
ELISA
C15.6
Unconjugated
Il12b
Purified
Il12b
IgG1
50μg
Store at 2-8°C.
Primary
16160
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